Sterilization Temperatures
In depth information lives on the pages for the individual methods:
Dry Heat Sterilization Temperatures1
| Temperature | Time |
|---|---|
| 170°C/338°F | 60 mins |
| 160°C/320°F | 120 mins |
| 150°C/302°F | 150 mins |
Dry Heat Depyrogenation Temperatures
| Temperature | Time |
|---|---|
| 250°C/482°F | 30 mins1 |
| 200°C/392°F | 60 mins2 |
Moist Heat Autoclave Sterilization Parameters3
| Temperature | 121°C |
| Pressure | 15 psi |
| Time | 20-60 minutes* |
*Youāre supposed to choose a time that you can verify is correctly sterilizing your product. Since thatās typically out of scope for DIY operations, you may choose to sterilize for the maximum recommended time. The CDC recommends 30 minutes4.
Instant Pot Sterilization Parameters5
| Setting | Pressure cook high or max |
| Time | 150 minutes |
References
View the library page for access to some PDFs.
Footnotes
-
USP 797 section 10.3, 2024 edition ā©
-
Assessment and verification of commercially available pressure cookers for laboratory sterilizationā ā©
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